「水稻高產優質性狀形成的分子機理及品種設計」項目榮獲2017年度國家自然科學一等獎(附相關文獻)

2018年1月8日,由國家最高科學技術獎獲得者李振聲院士推薦的「水稻高產優質性狀形成的分子機理及品種設計」項目榮獲2017年度國家自然科學一等獎。這項成果是李家洋、韓斌、錢前、王永紅、黃學輝為代表的研究團隊歷時逾20年合作完成,給予了品種設計育種研究領域新的啟迪,將極大推動作物傳統育種向高效、精準、定向的分子設計育種轉變。

該項目圍繞「水稻理想株型與品質形成的分子機理」這一核心科學問題,鑒定、創製和利用水稻資源,創建了直接利用自然品種材料進行複雜性狀遺傳解析的新方法;揭示了影響水稻產量的理想株型的分子基礎,發現了理想株型形成的關鍵基因;闡明了稻米食用品質精細調控網路,用於指導優質稻米品種培育;示範了高產優質為基礎的設計育種,攻克了水稻高產優質協同改良的科學難題。研究成果引領了水稻遺傳學的發展,實現了「綠色革命」新突破。

左起黃學輝、韓斌、李家洋、錢前、王永紅五位獲獎人合影

部分代表作如下(文末附下載鏈接):

1,Song X, Lu Z, Yu H, Shao G, Xiong J, Meng X, Jing Y, Liu G, Xiong G, Duan J, Yao XF, Liu CM, Li H, Wang Y, and Li J (2017). IPA1 functions as a downstream transcription factor repressed by D53 in strigolactone signaling in rice. Cell Res 27, 1128-1141.

2,Meng X, Yu H, Zhang Y, Zhuang F, Song X, Gao S, Gao C, and Li J (2017). Construction of a Genome-Wide Mutant Library in Rice Using CRISPR/Cas9. Mol Plant 10, 1238-1241.

3,Ma H, Duan J, Ke J, He Y, Gu X, Xu TH, Yu H, Wang Y, Brunzelle JS, Jiang Y, Rothbart SB, Xu HE, Li J, and Melcher K (2017). A D53 repression motif induces oligomerization of TOPLESS corepressors and promotes assembly of a corepressor-nucleosome complex. Sci adv 3, e1601217.

4,Hu X, Meng X, Liu Q, Li J, and Wang K (2017). Increasing the efficiency of CRISPR-Cas9-VQR precise genome editing in rice. Plant Biotechnol J doi.org/10.1111/pbi.127

5,Zhang L, Yu H, Ma B, Liu G, Wang J, Wang J, Gao R, Li J, Liu J, Xu J, Zhang Y, Li Q, Huang H, Xu J, Li J, Qian Q, Han B, He Z & Li J (2017) A natural tandem aray alleviates epigenetic repression of PA1 and leads to superior yielding in rce. Nat Commun 8, 14789.

6,Zeng D, Tian Z, Rao Y, Dong G, Yang Y, Huang L, Leng Y, Xu J, Sun C, Zhang G, Hu J, Zhu L, Gao Z, Hu X, Guo L, Xiong G, Wang Y, Li J, Qian Qian (2017) Rational design of high-yield and superior-quality rice. Nat Plants 3, 17031.

7,Wang J, Yu H, Xiong G, Lu Z, Jiao J, Meng X, Liu G, Chen X, Wang Y and Li J (2017) Tissue-specific Ubiquitination by IPA1 INTERACTING PROTEIN 1 Modulates IPA1 Protein Levels to Regulate Plant Architecture in Rice.The Plant Cell 29, 697-707.

8,Zhou H, Wang L, Liu G, Meng X, Jing Y, Shu X, Kong X, Sun J, Yu H, Smith SM, Wu D and Li J (2016)Critical roles of soluble starch synthase SSIIIa and granule-bound starch synthase Waxy in synthesizing resistant starch in rice. PNAS USA 113, 12844–12849.

9,Li J, Meng X, Zong Y, Chen K, Zhang H, Liu J, Li J and Gao C (2016) Gene replacement and insertions in rice by intron targeting using CRISPR/Cas9. Nat Plants DOI: 10.1038/NPLANTS.2016.139.

10,Huang S, Weigel D, Beachy RN, and Li J. (2016) A proposed regulatory framework for genome-edited crops. Nat Genet 48, 109-111.

11,Ke J, Ma H, Gu X, Thelen A, Brunzelle J, Li J, Xu H, and Melcher K. (2015). Structural basis for recognition of diverse transcriptional repressors by the TOPLESS family of corepressors. Science Adv 1, e1500107.

12,Hu J, Wang Y, Fang Y, Zeng,L, Xu J, Yu H, Shi Z, Pan J, Zhang D, Kang S, Zhu L, Dong G, Guo L, Zeng D, Zhang G, Xie L, Xiong G, Li J, and Qian Q. (2015). A Rare Allele of GS2 Enhances Grain Size and Grain Yield in Rice. Molecular Plant 8, 1455-1465.

13,Liu L, Tong H, Xiao Y, Che R, Xu F, Hu B, Liang C, Chu J, Li J, and Chu C. (2015). Activation of Big Grain1 significantly improves grain size by regulating auxin transport in rice. Proc Natl Acad Sci USA 112, 11102-11107.

14,Zhao L, Zhou X, Yi W, Wu Z, Liu Y, Kang Y, Hou L, de Waal P, Li S, Jiang Y, Scaffidi A, Flematti G, Smith S, Lam V, Griffin P, Wang Y, Li J, Melcher K, and Xu H (2015). Destabilization of strigolactone receptor DWARF14 by binding of ligand and E3-ligase signaling effector DWARF3. Cell Res 25, 1219-1236.

15,Wang Y, Xiong G, Hu J, Jiang L, Yu H, Xu J, Fang Y, Zeng L, Xu E, Xu J, Ye W, Meng X, Liu R, Chen H, Jing Y, Wang Y, Zhu X, Li J, Qian Q. (2015) Copy number variation at the GL7 locus contributes o grain size diversity in rice. Nat Genet 47, 944-948.

16,Jing H, Yang X, Zhang J, Liu X, Zheng H, Dong G, Nian J, Feng J, Xia B, Qian Q, Li J, and Zuo, J (2015). Peptidyl-prolyl isomerization targets rice Aux/IAAs for proteasomal degradation during auxin signalling. Nat Commun 6, 7395.

17,Lu Z, Shao G, Xiong J, Jiao Y, Wang J, Liu G, Meng X, Liang Y, Xiong G, Wang Y, and Li J (2015)MONOCULM 3, ortholog of WUSCHEL in rice, is required for tiller bud formation. J Genet Genomics 42. 71-78.

18,Huang X, Yang S, Gong J, Zhao Y, Feng Q, Gong Hao, Li W, Zhan Q, Cheng B, Xia J, Chen N, Hao Z, Liu K, Zhu C, Huang T, Zhao Q, Zhang L, Fan D, Zhou C, Lu Y, Weng Q, Wang Z, Li J & Han B (2015)Genomic analysis of hybrid rice varieties reveals numerous superior alleles that contribute to heterosis. Nat Commun 6, 6258.

19,Sang D, Chen D, Liu G, Liang Y, Huang L, Meng X, Chu J, Sun X, Dong G, Yuan Y, Qian Q, Li J, and Wang Y. (2014). Strigolactones regulate rice tiller angle by attenuating shoot gravitropism through inhibiting auxin biosynthesis. Proc Natl Acad Sci USA 111, 11199-11204.

20,Cardoso C, Zhang Y, Jamil M, Hepworth J, Charnikhova T, Dimkpa S, Meharg C, Wright M, Liu J, Meng X, Wang Y, Li J, McCouch S, Leyser O, Price A, Bouwmeester H and Ruyter-Spira C (2014) Natural variation of rice strigolactone biosynthesis is associated with the deletion of two MAX1 orthologs. Proc Natl Acad Sci USA 111: 2379–2384.

21,Jiang L, Liu X, Xiong G, Liu H, Chen F, Wang L, Meng X, Liu G, Yu H, Yuan Y, Yi W, Zhao L, Ma H, He Y, Wu Z, Melcher K, Qian Q, Xu H, Wang Y and Li J (2013) DWARF 53 acts as a repressor of strigolactone signaling in rice. Nature 504: 401-405.

22,Lu Z, Yu H, Xiong G, Wang J, Jiao Y, Guifu Liu G, Jing Y, Meng X, Hu X, Qian Q, Fu X, Wang Y and Li J (2013) Genome-wide binding analysis of the transcription activator IPA1 reveals a complex network regulating rice plant architecture. Plant Cell 25: 3743-3759.

23,Gao Z, Zhao S, He W, Guo L, Wang J, Peng Y, Guo X, Zhang X, Rao Y, Zhang C, Dong G, Zheng F, Lu C, Hu J, Zhou Q, Liu H,Hu X, Xu J, Zeng D, Liu D, Tian P, Gong L, Li Y, Zhang G, Wang J, Tian F, Xue D, Liao Y, Chen M,Li J, Cheng S, Zhang G, WangJ, Qian Q (2013) Dissecting yield-associated loci in super hybrid rice by resequencing recombinant inbred lines and improving parental genome sequences. Proc Natl Acad Sci USA110: 14492-14497.

24,Liu L, Shang-Guan K, Zhang B, Liu X, Yan M, Lanjun Zhang L, Shi Y, Zhang M, Qian Q, Li J and Zhou Y (2013) Brittle Culm1, a COBRA-Like Protein, Functions in Cellulose Assembly through Binding Cellulose Microfibrils. PLoS Genet 9: e1003704.

25,Zhao L, Zhou X, Wu Z, Yi W, Xu Y, Li S, Xu T, Liu Y, Chen R, Kovach A, Kang Y, Hou L, He Y, Xie C, Song W, Zhong D, Xu Y, Wang Y, Li J, Zhang C, Melcher K and Xu H (2013) Crystal structures of two phytohormone signal-transducing α/β hydrolases: karrikin-signaling KAI2 and strigolactonesignaling DWARF14. Cell Res 23:436-439.

26,Chen L, Xiong G, Cui X, Yan Y, Xu T, Qian Q, Xue Y, Li J and Wang Y (2013) OsGRAS19 may be a novel component involved in the brassinosteroid signaling pathway in rice. Mol Plant 6: 988-991.

27,Zhang X, Wang J, Huang J, Lan H, Wang C, Yin C, Wu Y, Tang H, Qian Q, Li J, and Zhang H (2012)Rare allele of OsPPKL1 associated with grain length causes extra-large grain and a significant yield increase in rice. Proc Natl Acad Sci USA 109: 21534-21539.

28,Huang X, Kurata N, Wei X, Wang Z, Wang A, Zhao Q, Zhao Y, Lu H, Li W, Guo Y, Lu Y, Liu K, Zhou C, Fan D, Weng Q, Zhu C, Huang T, Zhang L, Wang Y, Feng L, Furuumi H, Kubo T, Miyabayashi T, Yuan X, Xu Q, Dong G, Zhan Q, Li C, Fujiyama A, Toyoda A, Lu T, Feng Q, Qian Q, Li J and Han B (2012) A map of rice genome variation reveals the origin of cultivated rice and domestication-associated genes. Nature 490: 497-502.

29,Xu C, Wang Y, Yu Y, Duan J, Liao L, Xiong G, Meng X, Liu G, Qian Q and Li J (2012) Degradation of MONOCULM 1 (MOC1) by APC/CTAD1regulates rice tillering. Nat Commun DOI: 10.1038/ncomms1743.

30,Huang X, Zhao Y, Wei X, Li C, Wang A, Zhao Q, Li W, Guo Y, Deng L, Zhu C, Fan D, Lu Y, Weng Q, Liu K, Zhou T, Jing Y, Si L, Dong G, Huang T, Lu T, Feng Q, Qian Q, Li J and Han B (2012) Genome-wide association study of flowering time and grain yield traits in a worldwide collection of rice germplasm. Nat Genet 44: 32-39.

31,Huang X, Wei X, Sang T, Zhao Q, Feng Q, Zhao Y, Li C, Zhu C, Lu T, Zhang Z, Li M, Fan D, Guo Y, Wang A, Wang L, Deng L, Li W, Lu Y, Weng Q, Liu K, Huang T, Zhou T, Jing Y, Li W, Lin Z, Bucker ES, Qian Q, Zhang Q, Li J and Han B (2010) Genome-wide association studies of 14 agronomic traits in rice landraces. Nat Genet 42: 961-967.

32,Jiao Y, Wang Y, Xue D, Wang J, Yan M, Liu G, Dong G, Zeng D, Lu Z, Zhu X, Qian Q and Li J (2010)Regulation of OsSPL14 by OsmiR156 defines ideal plant architecture in rice. Nat Genet 42, 541-544.

33,Lin H, Wang R, Qian Q, Yan M, Meng X, Fu Z, Yan C, Jiang B, Su Z, Li J, and Wang Y (2009).DWARF27, an iron-containing protein required for the biosynthesis of strigolactones, regulates rice tiller bud outgrowth. Plant Cell 21: 1512-1525.

34,Huang X, Qian Q, Liu Z, Sun H, He S, Luo D, Xia G, Chu C, Li J, and Fu X. (2009) Natural variation at the DEP1 locus enhances grain yield in rice. Nat Genet 41: 494-497.

35,Li S, Qian Q, Fu Z, Zeng D, Meng X, Kyozuka J, Maekawa M, Zhu X, Zhang J, Li J and Wang Y (2009)Short panicle1 encodes a putative PTR transporter and determines rice panicle size. Plant J 58: 592-605.

36,Zhou Y, Li S, Qian Q, Zeng D, Zhang M, Guo L, Liu XL, Zhang B, Deng L, Liu XF, Luo G, Wang X and Li J (2009) BC10, a DUF266-containing and golgi-located type II membrane protein, is required for cell wall biosynthesis in rice (Oryza sativa L.) Plant J 57: 446-462.

37,Tian Z, Qian Q, Liu Q, Yan M, Liu X, Yan C, Liu G, Gao Z, Tang S, Zeng D, Wang Y, Yu J, Gu M and Li J (2009) Allelic diversities in rice starch biosynthesis lead to a diverse array of rice eating and cooking qualities. Proc Natl Acad Sci USA 106: 21760–21765.

38,Yu Y, Tang T, Qian Q, Wang Y, Yan M, Zeng D, Han B, Wu Chung-I, Shi S and Li J (2008)Independent losses of function in a polyphenol oxidase in rice: differentiation in grain discoloration between subspecies and the role of positive selection under domestication. Plant Cell 20: 2946-2959.

39,Li P, Wang Y, Qian Q, Fu Z, Wang M, Zeng D, Li B, Wang X, and Li J (2007) LAZY1 controls rice shoot gravitropism through regulating polar auxin transport. Cell Res 17: 402-410.

40,Xiong G, Hu X, Jiao Y, Yu Y, Chu C, Li J, Qian Q and Wang Y (2006) LEAFY HEAD2, which encodes a putative RNA-binding protein, regulates shoot development of rice. Cell Res 16: 267-276.

41,Li Y, Qian Q, Zhou Y, Yan M, Sun L, Zhang M, Fu Z, Wang Y, Han B, Pang X, Chen M and Li J (2003)BRITTLE CULM 1, which encodes a COBRA-like protein, affects the mechanical properties of rice plants. Plant Cell 15: 2020-2031.

42,Li X, Qian Q, Fu Z, Wang Y, Xiong G, Zeng D, Wang X, Liu X, Teng S, Hiroshi F, Yuan M, Luo D, Han B and Li J (2003) Control of tillering in rice. Nature 422: 618-621.

43,Feng Q, Zhang Y, Hao P, Wang S, Fu G, Huang Y, Li Y, Zhu J, Liu Y, Hu X, Jia P, Zhang Y, Zhao Q, Ying K, Yu S, Tang Y, Weng Q, Zhang L, Lu Y, Mu J, Lu Y, Zhang LS, Yu Z, Fan D, Liu X, Lu T, Li C, Wu Y, Sun T, Lei H, Li T, Hu H, Guan J, Wu M, Zhang R, Zhou B, Chen Z, Chen L, Jin Z, Wang R, Yin H, Cai Z, Ren S, Lv G, Gu W, Zhu G, Tu Y, Jia J, Zhang Y, Chen J, Kang H, Chen X, Shao C, Sun Y, Hu Q, Zhang X, Zhang W, Wang L, Ding C, Sheng H, Gu J, Chen S, Ni L, Zhu F, Chen W, Lan L, Lai Y, Cheng Z, Gu M, Jiang J, Li J, Hong G, Xue Y and Han B (2002) Sequence and analysis of rice chromosome 4. Nature 420: 316-320.


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