在國際空間站對微生物進行 DNA 測序需要哪些技術？需要注意什麼？

01-25

國際空間站微生物在太空「就地測序」-新聞-科學網

寫了么

MinION。

基於納米孔的測序儀器，小巧，很方便，也不貴。

疾控中心去現場取樣直接測序用的就是這個。

技術的話先用pcr做library，就是先把樣本切切碎然後放大。然後用一些手段測序，就目前的測序技術來說不論是nanopore還是microfluidics對重力都沒有硬性要求。硬要說難點：

1、太大的設備運上去不划算。

2、目前沒有太空測序的需求。

具體方案可以參考官方公布的protocols：

Operational Requirements and Protocols

Crew member retrieves a sample preparation kit, flow cell and genomic DNA or cell samples from cold stowage or Environmental Health Systems hardware. miniPCR and Surface Pro3 tablet are retrieved from stowage and setup. The crew member first micropipettes DNA or cells into miniPCR sample tubes containing the enzymes necessary to perform DNA amplification using miniPCR. The crew member then places the tubes within miniPCR and initiates the amplification procedure. Once amplification is complete and upon retrieving the samples from miniPCR, the crew member uses a traditional micropipette to add enzymes to the miniPCR sample tubes to clean-up the amplified product. After a short incubation at ambient ISS temperature the amplified DNA is prepared for sequencing. To put the DNA into a sequence-ready format, the crew member once again pipettes enzymes into the reaction tubes. Following a short incubation at ambient ISS temperature, the DNA is ready to be sequenced. The crew member loads the prepared sample in a flow cell after inserting the flow cell into the MinION sequencer. The sequencer runs for either 6 or 48 hours unattended and shuts itself down. When the run is finished, data transfer from the Surface Pro3 to Earth is initiated from the ground. The crew member then removes the flow cell from the sequencer and discards it.

畢竟設備早就小型化了，全套設備控制在在5000美金以內毫無壓力，和太空任務經費比起來基本可以忽略不計。

而且全傻瓜操作。

https://www.minipcr.com/

https://nanoporetech.com/products/minion

當然，不花錢就搞個第一還是不錯的，不出意外估計還能灌一篇一區論文，估計可以有20個以上的作者。

真是皆大歡喜啊。

去年帶了oxford nanopore minion測序儀到空間站測序了，17年年底文章發在sr上。

這個還真不清楚，具體的操作得問專業搞這方面研究的。不過既然是在國際空間站，應該還是要注意培養基的理化性質可能會受影響等等

測序可以帶回來測吧，拉個機器上去不容易啊，帶點培養基上去就好了，主要注意防止人為污染。

需要問航天人哦，我也非常感興趣這個問題

可以關注Christopher Mason的工作，他和NASA有緊密合作。測序儀用的是ONT，我司儀器可能帶不上飛船。